Cygnus公司産品涵蓋了E.Coli大腸杆菌宿主蛋白殘留、CHO細胞宿主蛋白殘留、HEK293細胞宿主蛋白殘留、Vero細胞宿主蛋白殘留以及細胞培養添加物如BSA殘留、Protein A殘留、insulin胰島素殘留等的檢測試劑盒。

 This ELISA incorporates some improvements over the Cat. #F050 and F050H kits and is designed to better detect the newer, unnatural constructs of Protein A such as the MabSelect SuReTM ligand sold by GE Healthcare. If you are using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM you should use the Catalog #F400 kit. Since this kit has been released, 2 newer Protein A assays, known as Mix-N-Go methods, have been developed. These new methods eliminate the sample boiling and centrifugation steps. Instead, a special sample treatment reagent is used that allows for accurate detection of Protein A in the presence of your sample antibody. The Cat. #F600 kit uses standards calibrated against natural and conserved recombinant forms of Protein A. The Cat. #F610 kit uses standards calibrated to unnatural constructs of Protein A such as GE Healthcare's MabSelect SuReTM.

F400 Protein A ELISA kit 蛋白AELISA檢測試劑盒 較之早期同類産品進(jìn)行了一些改進(jìn)以便更好(hǎo)地識别新型經(jīng)修飾的非天然結構蛋白，例如GE Healthcare出售的MabSelect SuReTM配體等。如果您使用的是重組蛋白，且與天然蛋白存在顯著的結構差異（如MabSelect SuReTM等），則應該選用此試劑盒，如果您是首次使用我們的蛋白A檢測産品，我們建議您選擇此試劑盒，因爲它的操作性、穩定性和精度都(dōu)高于老款試劑盒。檢出限~50pg/ml。我們改進(jìn)了樣(yàng)品處理步驟，減少了繁瑣的移液操作，從而提高了樣(yàng)本處理效率。新方法提高了檢測精度和穩定性。配有微孔闆離心機的實驗室現在可以同時(shí)處理多達96個樣(yàng)本，新産品用96孔PCR闆和密封貼代替了單個的離心管，新操作也最大限度減少了處理離心管時(shí)對(duì)顆粒狀變性蛋白的無意破壞。